Western blot analysis performed on HEK293 cell lysates infected with dual-intein EGFP LVs. Cell lysates from cells transduced with: 1. EGFP full-length LVs at MOI = 5; 2. dual-intein N-term EGFP LV at MOI = 5; 3. dual-intein C-term EGFP LV at MOI = 5; 4. dual-intein N-term and C-term EGFP LVs at MOI = 2.5 each vector; 5. dual-intein N-term and C-term EGFP LVs at MOI = 5 each vector; 6. untreated cells. Black arrows indicate the correct spliced EGFP produced from co-infection; blue and red arrows indicate the single halves produced by LVs expressing for the N-term and C-term EGFP, respectively; red boxes indicate the spliced intein.