TAQC substrates can be degraded by lysosomes and the proteasome. (A) ssGFP_K20-transfected cells were treated with Baf A1 (200 nM 4 h) and stained with LAMP1 antibodies (red). Note the partial localization of ssGFP_K20 with LAMP1. Scale bar, 5 μm. (B) ERGFP_NS-expressing HEK293T cells treated with the indicated compounds were pulse labeled with S35-Met and chase in a medium containing unlabeled S35-Met. Samples taken at the indicated time points were subject to immunoprecipitation and autoradiography. CQ, chloroquine. Source data are available for this figure: SourceData FS1.