Targeted elimination of FOLR2 ⁺ macrophages augments CNV. (A) Representative laser CNV lesions in vehicle and topical diphtheria toxin (DTx) mice 7 days after laser. GS-IB4 lectin stain (vasculature). Scale bar = 100 μm. (B) Quantification of laser CNV lesion size per eye. Areas were averaged over four lesions. n = 7 eyes per group. Student’s t test. (C) Myeloid quantifications from flow cytometry of vehicle and DTx CNV mice. Frequencies are relative to all myeloid cells. Mono. = monocytes; Neut. = neutrophils; MΦ = choroidal macrophages. Yellow = vehicle. Blue = DTx. n = 5 eyes for vehicle and n = 4 eyes for DTx. Two-way ANOVA with Holm–Sidak correction. (D) Representative flow cytometry of all myeloid cells from vehicle and DTx CNV mice showing monocyte and neutrophil gates. (E) Representative flow cytometry showing MHCII and FOLR2 staining in choroidal macrophages from vehicle and DTx CNV mice. (F) Choroidal macrophage quantifications from flow cytometry of vehicle and DTx CNV mice. Frequencies are relative to all macrophages. Yellow = vehicle. Blue = DTx. n = 5 eyes for vehicle and n = 4 eyes for DTx. Two-way ANOVA with Holm–Sidak correction. (G) Representative flow cytometry showing MHCII and CD11c staining in choroidal macrophages from vehicle and DTx CNV mice. (H) MHCII⁺CD11c⁺ and MHCII⁺CD11c⁻ quantifications from flow cytometry of vehicle and DTx CNV mice. Frequencies are relative to all macrophages. Yellow = vehicle. Blue = DTx. n = 5 eyes for vehicle and n = 4 eyes for DTx. Two-way ANOVA with Holm–Sidak correction. Vehicle and DTx-treated mice from C–H are the same cohorts shown in Fig. S5 A, along with an additional control cohort treated with DTx under the same experimental conditions. (I) Multiplex immunofluorescence of CNV sprout from AMD donor. Maximum intensity projections are shown. Scale bars = 100 μm. (J) 3D rendering of neovascular sprout from I. Scale bar = 100 μm. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.