Diphtheria toxin control studies. (A) Flow cytometry from digested mouse choroids in LYVE1^Cre(−) × CSF1R^DTR(+) mice treated with either vehicle (yellow) or diphtheria toxin (DTx; magenta) compared with LYVE1^Cre(+) × CSF1R^DTR(+) mice treated with DTx (blue). Vehicle-treated LYVE1^Cre(−) × CSF1R^DTR(+) control mice and DTx-treated LYVE1^Cre(+) × CSF1R^DTR(+) depletion mice are the same cohorts from Fig. 6, C–H. DTx-treated LYVE1^Cre(−) × CSF1R^DTR(+) mice are an additional control cohort under the same experimental conditions. All mice were 1 mo old and treatments were for seven consecutive days (7 days) before and after laser CNV. Box plots show the frequencies of macrophage (MФ) subsets (MHCII⁺FOLR2⁺, MHCII⁻FOLR2⁺, and MHCII⁺FOLR2⁻) relative to all macrophages for each of the three experimental groups. Flow cytometry plots are pre-gated on macrophages and show the macrophage subsets. One-way ANOVA with Holm–Sidak correction. ** <0.01; *** <0.001; **** <0.0001. (B) OCT of LYVE1^Cre(−) × CSF1R^DTR(+) mice before and after treatment with DTx for seven consecutive days. Four independent biological replicates. The same area of the optic nerve head is shown for the before and after DTx images. Scale bar = 100 μm.