scRNA-seq myeloid marker genes and flow cytometry gating schemes for human and mouse choroidal digests. (A) Human flow cytometry gating scheme. Boxed area shows fluorescence minus one (FMO) controls for the respective gating steps. (B) scRNA-seq representation of the human flow cytometry gating scheme showing RNA expression of the flow cytometry target antigens for each gating step. (C) Marker genes for myeloid clusters from human choroid scRNA-seq. (D) Mouse choroid flow cytometry gating scheme. MФ, macrophage.