PRC2 contributes to Aire-mediated gene expression and the development of mimetic cells. Related to Fig. 6. (A) t-SNE visualization of 2,552 TECs. Cells sorted as mTEC^high (CD45⁻EpCAM⁺Ly51⁻MHC-II^high) are color-coded according to mouse age and genotypes. Cell numbers for each group are indicated on the top. (B) Confusion matrix indicating the corresponding clusters for Fig. 6 A (query) in scMulti-seq (reference) shown in Fig. 1 A, with normalization for each column (query). (C) FC-FC plot comparing gene regulation during mTEC maturation (x axis) and PRC2-mediated gene regulation (y axis), with a green trendline representing the SMA regression. Pearson’s correlation coefficient and P value by the F-test are indicated at the top. (D) GO analysis of DEGs predominantly up-regulated in each cluster shown in Fig. 6 G. P values from the hypergeometric distribution were BH-corrected. (E) FC-FC plot comparing gene regulation during mTEC maturation (x axis) and PRC2-mediated gene regulation (y axis), with the mimetic cell genes from Fig. 6 G highlighted in red (see also Fig. 6 H). (F) Box plots indicating the difference in H3K27me3 density in mTEC^low and mTEC^high from Aire-KO for gene loci ± 10-kb regions of mimetic cell genes up-regulated in the indicated clusters (see also Fig. 6 G), determined by CUT&Tag (n = 2 for each).