Effect of recovery time before selection on antibiotics. (A) Number of Hyg^R transformants from four independent transformations with cells recovered in 50% YPD with 0.5 M sorbitol for the indicated times prior to selection. (B) Cell growth is quantified at the indicated times by counting the number of cells/ml. (C) The percent of colonies on Hyg. B out of the total cells in 310 µl plated at the indicated times. (D) % of correct ura3::HYG^R integrants for each condition in A. (E) Number of Hyg^R transformants from three or four independent transformations using DNA encoding the hygromycin resistance cassette flanked by sequences of different lengths that are homologous to the URA3 locus. (F) % of correct ura3::HYG^R integrants from the experiments in E. In all graphs, the mean and standard deviation are shown. Statistical significance calculated by one-way ANOVA with P values indicated (not shown, P > 0.05; *, P ≤ 0.05; ***, P ≤ 0.001; ****, P ≤ 0.0001).