Hierarchical clustering defines two groups of genetically diverse mice with divergent CD4 T cell profiles. (A) Heatmap depicting immune features measured from the lungs of three female mice per strain from one experiment at 4 wk after infection. All features were scaled and centered with rows and columns hierarchically clustered via complete linkage method. (B) Lung bacterial burden (log₁₀ transformed). (C) IFNγ levels from total lung homogenate as measured by multiplex Luminex assay. (D) Percent of lung CD4 T cells staining positive by flow cytometry for the transcription factors T-bet, Foxp3, or GATA3. (B–D) Box-and-whisker plots depict bounds from the 25th to 75th percentile, median line, and whiskers extending from min to max with all points shown (each point is an individual mouse). Statistical significance was assessed via Mann–Whitney U test with Benjamini–Hochberg correction (*P < 0.05, **P < 0.01). NK, natural killer cell; DC, dendritic cell.