Loss of Abl alters Notch co-localization with endosomal markers. (A–F″) Single confocal sections showing Notch and endosomal markers in 32 h APF WT and abl^null wings. (A and B) anti-Notch and anti-Eps15. (C and D) anti-Notch and anti-Hrs. (E and F) anti-Notch and endogenous Rab7-EYFP. Yellow arrowheads point to examples of Notch-endosomal marker co-localization. Scale bars = 5 µm. (G) Pearson’s coefficient–based co-localization analysis. Each dot represents the Pearson’s coefficient from a group of ∼100 cells in an individual wing. N = 24 different wings were evaluated per condition. A t-student means comparison test was performed, and P values <0.05 (*), <0.01 (**), <0.001(***), and <0.0001 (****) are indicated.