Decreased G2/M cell cycle phase following the reduction in BRCA1 and cisplatin treatment and decreased proliferation following BRCA1 reduction in vitro. (A) Cell cycle analysis, using a flow cytometer, of HKC8 cells (transduced with shCtrl, shBRCA1–1, or shBRCA1–2) treated with 0 µM cisplatin, 25 µM cisplatin, and 0 µM B02 (a RAD51 inhibitor); 25 µM cisplatin and 5 µM B02 (third panel); and 0 µM cisplatin and 5 µM B02. n = 3 per condition. (B and C) (B) HK2 and (C) HKC8 cells were transfected with Ctrl siRNA (siCtrl) or BRCA1 siRNAs (siBRCA1 #6 and siBRCA1 GUU), and the number of viable cells was counted using trypan blue dye exclusion method. The number of cells at 24 and 96 h after transfection was plotted. n = 3 per condition. Experiments were performed in triplicate and repeated two times.