Phagosomal resolution is driven by sensitivity of ClC-7 to PI(3,5)P ₂ . (A) WT and Snx10 KO RAW 264.7 macrophages were either untransfected (top row) or transiently transfected with either ClC-7-GFP or ClC-7^Y715C-GFP 12 h before being challenged with IgG-coated sRBCs. Imaging to assess the degradation of the engulfed targets was performed 4 h after phagocytosis, using anti-IgG antibodies to visualize the opsonin. (B) Mean sRBC volumes measured 4 h after phagocytosis in experiments like those illustrated in A. Data from three independent experiments, each representing >25 phagosomes.