Phospholipid content of WT and Snx10 KO cells. (A–H) Levels of (A) PC, (B) PE, (C) PS, (D) PG, (E) PA, (F) PI, (G) phosphatidylinositol 4-phosphate (PI4P), (H) PI(4,5)P₂, and (I) phosphatidylinositol 3,4,5-trisphosphate (PIP₃) as determined by PRMC-MS. Data from three independent determinations. PRMC-MS: phosphoinositide regioisomer measurement by chiral column chromatography and mass spectrometry.