Delivery of ClC-7 to mature phagosomes does not require Snx10. (A) WT, ClC-7 KO, and Snx10 KO RAW 264.7 macrophages were challenged with IgG-opsonized silica beads for 30 min prior to fixing. Cells were then stained with anti-ClC-7 antibodies (red), phalloidin (Acti-Stain 488; cyan), and anti-IgG antibodies (white in insets). (B) Mean ClC-7 signal intensity on phagosomes, measured in three independent experiments. MFU = mean fluorescence units.