Phagosomal maturation and acidification occur independently of Snx10. (A) WT and Snx10 KO RAW 264.7 cells challenged with IgG-opsonized sRBCs for 3 or 30 min, then immunostained with anti-LAMP1 antibodies and visualized with fluorescent secondary antibodies against the antibodies opsonizing the sRBCs (green) and the anti-LAMP1 antibodies (magenta). (B and C) WT and Snx10 KO RAW 264.7 cells were challenged with IgG-opsonized, FITC-conjugated sRBCs (B), or zymosan particles (C) for 1 h prior to imaging. The steady-state pH of >100 phagosomes from three independent experiments is shown. Where noted, 500 nM of concanamycin A (CCA) was used for 30 min prior to imaging to neutralize phagosomal pH.