Snx10 is required for proper degradative capacity of phagosomes and lysosomes. (A) Lysates of WT and two separate Snx10 KO RAW 264.7 cell clones were probed with anti-Snx10 and anti-vinculin antibodies (used to normalize loading) and analyzed by SDS-PAGE. Molecular weight markers (kDa) are indicated. (B and C) WT and Snx10 KO RAW 264.7 cells challenged with IgG-coated sRBCs and visualized 1 or 4 h after phagocytosis with anti-IgG antibody (magenta). Images in B are representative of three independent experiments. Mean ± SD of sRBC phagosome volumes were quantified at 1 and 4 h in C. n = 3 independent experiments, each counting >70 phagosomes. (D) Representative fluorescence images of WT and Snx10 KO RAW 264.7 cells challenged with IgG- and DQ-BSA–coated silica beads for 30 min. DQ-BSA signal is presented in pseudocolor. (E) Quantification of relative DQ-BSA fluorescence intensity in WT and Snx10 KO RAW 264.7 cells. n = 3, each independent experiment measuring >25 phagosomes. (F) Lysates of WT and Snx10 KO cells probed with anti-cathepsin C (Cath C) antibody and anti-vinculin antibody used to normalize loading. The position of pro-cathepsin and mature (cleaved) cathepsin are indicated. Molecular weight markers (kDa) indicated on left. (G) Quantification of mature cathepsin C levels relative to the total cathepsin C. n = 3.