BATF overexpression does not fully rescue the SLEC differentiation transcriptional program without Notch signaling. (A) Differentiation of BATF overexpressing OT-I cells into CX3CR1^hiCXCR3^lo SLECs. BATF overexpression and OT-I T cell response were done as in Fig. 7. Representative CXCR3 versus CX3CR1 plots gated on OT-I SLECs. (B) Compilation of CX3CR1^hiCXCR3^lo SLECs as shown in A. (C) Venn diagram illustrating the overlap of the Notch (WT empty-RV versus Notch KO empty-RV), BATF (WT versus KO), and BATF overexpression in Notch KO (Notch KO BATF-RV versus Notch KO empty-RV). (D) BATF overexpression in Notch KO day 3 effector CD8⁺ T cells does not lead to enrichment of the SLEC signature. NES, normalized enrichment score; FDR, false discovery rate. Data are from two independent experiments (A and B) with a total of n = 8 mice per group. Repeated measures two-way ANOVA with Sidak’s multiple comparison test was used for GFP⁻ versus GFP⁺ comparisons. **P < 0.01, ****P < 0.0001.