LET-2/EMB-9 translation reporter ratios in tissues mirror BM ratios. (A) Top: a schematic diagram of a late L4 larva with tissues analyzed for LET-2 and EMB-9 translation reporters shown. Bottom: single z-slice confocal fluorescence images of translation reporters for LET-2 (let-2::P2A::PEST::mNG) and EMB-9 (emb-9::P2A::PEST::mNG) in the body wall muscle, spermatheca, and DTC in late L4 larvae. Yellow dotted lines indicate where measurements were taken. The scale bar is 20 μm. (B) Top: boxplot of the mean fluorescence intensity measurements of the LET-2 translational reporter normalized to the average of all mean fluorescence intensity measurements of the EMB-9 translational reporter in the same tissue and trial (see Materials and methods, n > 10 animals for each of three trials. ****P > 0.0001, ns, not significant, Kruskal–Wallis test followed by Dunn’s multiple comparisons test). Colors of the data points indicate different trials. Bottom: table showing the ratios of the average fluorescence levels of LET-2 translation reporter to EMB-9 translation reporter in each trial. Colors in the table correspond to data points in the boxplot.