LET-2 is approximately twice as abundant as EMB-9 in larva, and the total EMB-9 and LET-2 proteins are comparable between strains. (A and B) Boxplots display the abundance of mNG and mRuby2 represented by their respective log₂ protein intensity (a.u.) determined using LC-MS/MS. The samples displayed are the larval (L1–L3 stages) cellular fractions of Strain 1 (EMB-9::mRuby2 [C]; LET-2::mNG [C]) and Strain 2 (EMB-9-mNG [C]; LET-2::mRuby2 [C]). The log₂ fold changes from the comparison between strains are listed above the boxplots. The mNG (LET-2) in Strain 1 (n = 3) was ∼1.7 times the abundance of mNG (EMB-9) in Strain 2 (n = 4), while mRuby2 (LET-2) was ∼1.7 times more abundant in Strain 2 (n = 3) than mRuby2 (EMB-9) in Strain 1 (n = 2). (C and D) Boxplots display the abundance of EMB-9 and LET-2 represented by their respective log2 protein intensity (a.u.). The samples displayed are the larval matrix fractions of Strain 1 and Strain 2 (n = 5 each). Log₂ fold changes from the comparison between strains are listed as well.