Tuft cell–specific NAIP–NLRC4 inflammasome activation mediates control of Salmonella Typhimurium infection in the small intestine. iNLRC4-Pou2f3-cre^ERT2mice or Nlrc4^−/− littermate controls were pretreated with oral gavage of streptomycin, then 24 h later infected with 5 × 10⁷ CFUs of Salmonella Typhimurium ΔSsaR by oral gavage. (A–D) Tissue CFUs from the small intestine (A) and cecum (B) 18 h after infection and small intestine (C) and cecum (D) 5 days after infection. (E) Number of Nlrc4^−/− or iNLRC4-Pou2f3-cre^ERT2 mice that had no detectable CFUs at day 5 after infection. (F and G) IL-22 protein levels were determined by ELISA in small intestine (F) or cecum (G). Each data point represents one mouse, pooled data from multiple experiments (n = 9 [A and B], n = 14–20 [C–G]), bars shown as the median. Significance was determined by Mann–Whitney U test (A–D, F, and G). *, P < 0.05, **, P < 0.01.