Tuft cells are necessary for an antimicrobial signature but tuft cell–PGD₂–ILC3 signaling is not required for Salmonella defense. (A–C) Gpr44 ^fl/flRorγt-Cre or Gpr44^fl/+Rorγt-Cre mice were infected through oral gavage with 5 × 10⁷ CFUs of Salmonella Typhimurium ΔSsaR. All mice were pretreated with streptomycin 24 h prior to the infection. Tissue CFUs from small intestine (ileum) (A) or cecum (B) 4 days after infection. (C) Flow cytometry of Gpr44-GFP Lin⁻CD90⁺CD127⁺KLRG1⁻NK1.1⁻Rorγt⁺ IL22 expression. (D–F) Mice deficient or sufficient in tuft cells were infected through oral gavage with 5 × 10⁷ CFUs of Salmonella Typhimurium ΔSsaR. Tissue CFUs from the small intestine (ileum) (D) or caecum (E) as well as quantification of tissue expression of Reg3γ mRNA (F) 5 days after infection. Each data point represents one mouse, pooled data from multiple experiments (n = 10–13 [A and B], n = 8 [C], n = 14–18 [D and E], n = 6–18 [F]), and bars shown as median. Mann–Whitney test was performed to determine significance. *, P < 0.05.