Effects of KLF2 deficiency on myeloid cells in the pleural and peritoneal cavities. (A) Flow cytometry gating strategy for cavities. (B) Flow cytometry of cells in pleural cavity lavage from the indicated mice, gated on live, single, CD3⁻B220⁻CD19⁻ cells. Numbers adjacent to gates indicate the percentage of total live events for each gate. (C) Bar graphs depicting the percentage of total live cells and total number of cells for the following populations in the pleural cavities (representative gates shown in A) as measured by flow cytometry: CD11b⁺F4/80⁺, SCM (CD11b⁺F4/80⁺MHCII^hiDNAM-1^hi), LCMs (CD11b⁺F4/80⁺MHCII⁻DNAM-1⁻), ICAM2⁺GATA6⁺ LCMs (CD11b⁺F4/80⁺MHCII⁻DNAM-1⁻ICAM2⁺GATA6⁺), and transitional (CD11b⁺F4/80⁺MHCII^midDNAM-1^mid). (D) Bar graphs depicting the percent of total and total number of monocytes (CD11b⁺F4/80⁻Ly6C⁺MHCII⁺ or MHCII⁻) and neutrophils (CD11b⁺F4/80⁻, Ly6G⁺), as measured by flow cytometry. (E and F) FlowSOM and ClusterExplorer-derived unsupervised cluster identification (E) and heatmap of marker intensities defining identified clusters (F). Significance determined by ordinary two-way ANOVA with multiple comparisons and Šidák’s correction. Asterisks denote: ****P < 0.0001, ***P = 0.0006, **P = 0.0021, and *P = 0.033.