AA induces Hog1/Jlp1 punctate foci that associate with Pab1-containing SGs. (A and B) WT cells (DL3187) co-expressing CFP-C-Hog1 (p3217), Jlp1-CFP-N (p3683), and Pab1-mCherry (p3681) were not stressed (A) or treated with AA (B). SGs were isolated from cell lysates (lower panels). The Merge panel for the image of granules corresponds to the inset boxes. Scale bars for images of cells are 1 μm and for images of lysates are 5 μm.