Fluorescence micrographs of SG protein co-localizations. (A) Hog1-GFP and Pab1-mCherry granules isolated from WT cells treated with AA. Values are mean granules detected per visual field ± standard deviation. (B) Hog1/Jlp1 Cyan BiFC and Pab1-mCherry granules isolated from WT cells treated with AA. Co-localization was carried out using Zen 64 software to identify signal overlap. (C) BiFC negative control images of cells expressing Jlp1-CFP-N (p3683) with CFP-C vector (p3199) in the absence of stress visualized by fluorescence (left) or visible light (DIC; right). (D) Hog1-GFP and Pbs2-mCherry granules isolated from cells treated with AA.