Figure 4.
AA treatment induces the assembly of Pab1-containing SGs, to which Hog1 associates. (A) WT cells (DL3187) expressing Pab1-mCherry (p3681) were treated with AA (10 min) ± pre-treatment with CHX (30 min). SGs were isolated from lysates of cells treated as above (lower panels). Scale bars for images of cells are 1 μm and for lysates are 5 μm. (B) WT cells expressing Hog1-GFP (p3680) were treated with AA. (C) Hog1-GFP and Pab1-mCherry granules. WT cells co-expressing Hog1-GFP (p3680) and Pab1-mCherry (p3681) were treated with AA or unstressed, and SGs were isolated from cell lysates. (D) Hog1-GFP and Pbs2-mCherry granules. WT cells (upper panels) co-expressing Hog1-GFP (p3680) and Pbs2-mCherry (p3686) were treated with AA or unstressed, and SGs were isolated from cell lysates. Scale bars in C and D are 5 μm, and the Merge panel corresponds to the inset boxes. Refer to the image caption for details.

AA treatment induces the assembly of Pab1-containing SGs, to which Hog1 associates. (A) WT cells (DL3187) expressing Pab1-mCherry (p3681) were treated with AA (10 min) ± pre-treatment with CHX (30 min). SGs were isolated from lysates of cells treated as above (lower panels). Scale bars for images of cells are 1 μm and for lysates are 5 μm. (B) WT cells expressing Hog1-GFP (p3680) were treated with AA. (C) Hog1-GFP and Pab1-mCherry granules. WT cells co-expressing Hog1-GFP (p3680) and Pab1-mCherry (p3681) were treated with AA or unstressed, and SGs were isolated from cell lysates. (D) Hog1-GFP and Pbs2-mCherry granules. WT cells (upper panels) co-expressing Hog1-GFP (p3680) and Pbs2-mCherry (p3686) were treated with AA or unstressed, and SGs were isolated from cell lysates. Scale bars in C and D are 5 μm, and the Merge panel corresponds to the inset boxes.

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