Co-IP of selected proteins with Hog1-Myc. (A– E ) WT cells (DL3187) co-expressing Hog1-Myc (p3673) and one of the following: (A) Jlp1-HA (p3676), (B) Sam1-HA (p3677), (C) Kap123-HA (p3678), (D) GST-Sec53 (p3649), or (E) Dur12-HA (p3679) were treated with AA (100 mM, pH 4.5 for 10 min). Anti-Hog1 IPs of extracts were separated by SDS-PAGE and subjected to immunoblot analysis. Molecular mass markers (in kDa) are shown on the right. The fold increase over signal from unstressed cells (C) was calculated relative to Hog1-Myc IP signal. Source data are available for this figure: SourceData FS1.