Figure 4. Simulation of Ca 2 release... | Rockefeller University Press

Figure 4.

Simulation of Ca2+release during Ca2+waves where dyads had exponentially distributed RyR2 cluster size. (A) Schematic of the model cell (dotted prism). The simulation was carried out in one quadrant of the cell (solid prism) where the intracellular boundaries were reflecting (i.e., no diffusion), as well as the end boundary marked by the black dot. The solid prism comprised a 33 × 33 × 222 array of voxels (cubes with sides of 0.25 μm). (B) Fluorescence is shown in the x–z plane (8 × 55 μm lying 1 μm above the cell midplane) at 100-ms intervals starting from 3 s after the start of the simulation. The Ca2+ wave is represented by the red area moving from right to left. The cyan circular patches represent Ca2+ sparks. The black dot in the plot at 3.6 s is a reference point to illustrate the orientation of the simulation output in A. The horizontal dashed lines show the position of the line scans that are commonly used experimentally and in Fig. 6 to chart the time course of Ca2+ release. Refer to the image caption for details.

Simulation of Ca ²⁺ release during Ca ²⁺ waves where dyads had exponentially distributed RyR2 cluster size. (A) Schematic of the model cell (dotted prism). The simulation was carried out in one quadrant of the cell (solid prism) where the intracellular boundaries were reflecting (i.e., no diffusion), as well as the end boundary marked by the black dot. The solid prism comprised a 33 × 33 × 222 array of voxels (cubes with sides of 0.25 μm). (B) Fluorescence is shown in the x–z plane (8 × 55 μm lying 1 μm above the cell midplane) at 100-ms intervals starting from 3 s after the start of the simulation. The Ca²⁺ wave is represented by the red area moving from right to left. The cyan circular patches represent Ca²⁺ sparks. The black dot in the plot at 3.6 s is a reference point to illustrate the orientation of the simulation output in A. The horizontal dashed lines show the position of the line scans that are commonly used experimentally and in Fig. 6 to chart the time course of Ca²⁺ release.