Figure S2. Dependence of RyR2 unitary... | Rockefeller University Press

Figure S2.

Dependence of RyR2 unitary current on luminal [Ca2+]. (A) Single RyR2 recordings at 0-mV bilayer potential in the presence of a cytoplasmic solution containing 1 mM Mg2+, 0.1 mM Ca2+, and 5 mM caffeine and a luminal solution containing 1 mM Mg2+ and the indicated [Ca2+]. Channel openings are shown as downward transitions from the baseline (dashed line). (B) Mean unitary current amplitude (n = 3) fitted with a Michaelis–Menten curve. Refer to the image caption for details.

Dependence of RyR2 unitary current on luminal [Ca ²⁺ ]. (A) Single RyR2 recordings at 0-mV bilayer potential in the presence of a cytoplasmic solution containing 1 mM Mg²⁺, 0.1 mM Ca²⁺, and 5 mM caffeine and a luminal solution containing 1 mM Mg²⁺ and the indicated [Ca²⁺]. Channel openings are shown as downward transitions from the baseline (dashed line). (B) Mean unitary current amplitude (n = 3) fitted with a Michaelis–Menten curve.