Characterization of the Rac proteins during apical constriction. (A) Micrographs from time-lapse movies depicting localization of WAVE and Arp2/3 in control and mig-2 RNAi-treated embryos from a lateral view. The diagrams underneath each micrograph highlight the observed Arp2/3 localization in E and neighboring cells. (B) Violin plots reporting changes in WAVE and Arp2/3 localization at Ep-P₄ contacts, other cell–cell contacts, and the cytoplasm upon RNAi depletion of MIG-2 (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n = 10 embryos). (C) Micrographs from time-lapse movies depicting localization of mNeonGreen::CED-10 from a lateral view. White arrowheads point to Ea and Ep cells. (D) DIC (up) and fluorescence (down) micrographs of stage-matched wild-type, knock-in, and ced-10 RNAi-treated embryos mounted side-by-side from a lateral view. (E) Violin plot depicting normalized relative intensity measurements of whole embryos from wild-type, knock-in, and ced-10 RNAi-treated embryos, with average fluorescence intensity in wild-type embryos set to 0% and knock-in embryos set to 100% (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n = 11 embryos). Statistical tests for experiments in B and E were chosen based on the normality and variance of the data. (B) For Ep-P4 contacts (control versus RNAi) and other cell contacts (control versus RNAi), Mann–Whitney test; for cytoplasm (control versus RNAi), unpaired t test. (E) Welch’s ANOVA followed by post-hoc Dunnett’s tests. Scale bar: 5 µm.