WAVE RNAi did not affect E-cadherin levels at cell–cell contacts. (A) Micrographs from time-lapse movies depicting localization of E-cadherin in control and WAVE RNAi-treated embryos from a lateral view. Scale bar: 5 µm. White arrowheads point to Ea and Ep cells. (B) Violin plots reporting changes in E-cadherin levels at Ep-P₄ contacts, non-Ep-P₄ cell–cell contacts, and Ea–MSxx contacts upon RNAi depletion of WAVE (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n = 10 embryos). Unpaired t test was used in B based on the normality and variance of the data.