WAVE regulates F-actin levels at multiple cell–cell contact regions. (A) Micrographs from time-lapse movies depicting localization of F-actin in control and WAVE RNAi-treated embryos from a ventral view. Scale bar: 5 µm. Green arrows, MS front (Ea-MSxx contact); yellow arrows, MS back (MSxx-ABxxxx); pink arrows, Ea–Ep contact; purple arrows, Ea/p-ABxxxx contacts; grey arrows, Ep–P₄ contact. White arrowheads point to Ea and Ep cells. (B) Violin plots reporting changes in F-actin levels at MS front, MS back, Ea–Ep contact, Ea/p–ABxxxx contacts, and Ep–P₄ contact upon RNAi depletion of WAVE (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n = 10 embryos). (C) Violin plots reporting changes in the ratio of F-actin at the front versus back of the MSxx cell (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n = 10 embryos). Statistical tests for experiments in B and C were chosen based on the normality and variance of the data. (B) Unpaired t test. (C) Mann–Whitney test. **P < 0.01, ****P < 0.0001.