Quantification of fluorescence in wild-type/knock-in/RNAi-treated embryos permits verification of knockdown effectiveness. (A) DIC (upper) and fluorescence (lower) micrographs of stage-matched wild-type, knock-in, and RNAi-treated embryos mounted side-by-side from a lateral view. Scale bar: 5 µm. (B) Violin plots depicting normalized relative intensity measurements of whole embryos from wild-type, knock-in, and RNAi-treated embryos. The average fluorescence intensity in wild-type embryos is set to 0%, and in knock-in embryos is set to 100%. Statistical tests for experiments in B were chosen based on the normality and variance of the data: for WAVE and WASH, one-way ANOVA followed by post-hoc Tukey’s tests; for WASP, Welch’s ANOVA followed by post-hoc Dunnett’s tests (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n ≥ 10 embryos).