Partial rescue of dEsyt KO phenotypes by dEsyt CaBM . (A) Representative optical neutralization (ON) images showing the rhabdomere structure of the indicated genotypes. Rearing conditions and the age of the flies are indicated on top. (*CL–Constant Light). Scale bar: 20 pixels (corresponds to ∼5 µm). (B) Quantification of the time course taken for retinal degeneration. 50 ommatidia were scored from five flies of each genotype and plotted. (N = 5; n = 50). (C) Confocal images showing the localization of RDGB in Rh1>; dEsytKO, Rh1>dEsyt::GFP; dEsytKO and Rh1>dEsytCaBM::GFP; dEsytKO photoreceptors of flies which are(i) 1-day-old dark reared (ii) 6-day-old- exposed to constant illumination (5,000 Lux). For (i) and (ii), RDGB visualized is detected using an antibody against the endogenous protein (Cyan). Rhabdomeres are outlined using phalloidin which marks F-actin (Red). A single ommatidium is shown. Scale bar: 2 µm.
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