Figure S1.
TANGO2 isoform alignment, location in U2OS cells, and purification in HEK293 cells. (a) In silico sequence alignments of the full-length TANGO2 isoforms 1 (UniProt ID: Q6ICL3-1), 2 (UniProt ID: Q6ICL3-2), and 5 (UniProt ID: Q6ICL3-5), using T-COFFEE server. Asterisks (*) indicate fully conserved residues, while a colon (:) and a period (.) represent strongly and weakly similar properties in the amino acid sequence. (b) Multiple sequence alignment of the 40 amino-terminal residues of TANGO2 orthologs in Homo sapiens, Danio rerio, Drosophila melanogaster, Caenorhabditis elegans, and Saccharomyces cerevisiae, using T-COFFEE software. The conserved regions LIL (green) and NRDE (orange) are highlighted. (c–e), U2OS cells transfected with TANGO2.Iso1-mScarlet (c), or the mutants TANGO2.∆LIL-mScarlet (d) and TANGO2.∆NRDE-mScarlet (e) (magenta) were incubated with the LD marker Bodipy Green and the mitochondrial marker MitoTracker Deep Red (cyan). Squares indicate the magnification area (inset). Scale bars = 10 µm. (f) Representative Coomassie staining gel of TANGO2.Flag purification. Lanes were loaded with samples of molecular marker (1, in kDa), cell lysate (2), pellet (3), flow-through fraction after bead binding (4), flow-through fraction after ATP wash (5), first elution (6), second elution (7), third elution (8), and beads (9). (g) Representative Coomassie staining gel of TANGO2.∆NRDE.Flag purification. Lanes were loaded with samples of cell lysate (1), flow-through fraction after ATP wash (2), molecular marker (3, in kDa), first elution (4), second elution (5), and beads (6). Source data are available for this figure: SourceData FS1.

TANGO2 isoform alignment, location in U2OS cells, and purification in HEK293 cells. (a) In silico sequence alignments of the full-length TANGO2 isoforms 1 (UniProt ID: Q6ICL3-1), 2 (UniProt ID: Q6ICL3-2), and 5 (UniProt ID: Q6ICL3-5), using T-COFFEE server. Asterisks (*) indicate fully conserved residues, while a colon (:) and a period (.) represent strongly and weakly similar properties in the amino acid sequence. (b) Multiple sequence alignment of the 40 amino-terminal residues of TANGO2 orthologs in Homo sapiens, Danio rerio, Drosophila melanogaster, Caenorhabditis elegans, and Saccharomyces cerevisiae, using T-COFFEE software. The conserved regions LIL (green) and NRDE (orange) are highlighted. (c–e), U2OS cells transfected with TANGO2.Iso1-mScarlet (c), or the mutants TANGO2.∆LIL-mScarlet (d) and TANGO2.∆NRDE-mScarlet (e) (magenta) were incubated with the LD marker Bodipy Green and the mitochondrial marker MitoTracker Deep Red (cyan). Squares indicate the magnification area (inset). Scale bars = 10 µm. (f) Representative Coomassie staining gel of TANGO2.Flag purification. Lanes were loaded with samples of molecular marker (1, in kDa), cell lysate (2), pellet (3), flow-through fraction after bead binding (4), flow-through fraction after ATP wash (5), first elution (6), second elution (7), third elution (8), and beads (9). (g) Representative Coomassie staining gel of TANGO2.∆NRDE.Flag purification. Lanes were loaded with samples of cell lysate (1), flow-through fraction after ATP wash (2), molecular marker (3, in kDa), first elution (4), second elution (5), and beads (6). Source data are available for this figure: SourceData FS1.

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