Figure S9.
Co-immunoprecipitation analysis of HYLS1 with CPAP and CEP120. (A) Representative co-immunoprecipitation of Myc-EGFP-CPAP in HYLS1−/− DLD1 cells with HYLS1-mCherry (WT or D211G) add-back. Cells were induced with doxycycline for 4 days and Myc-EGFP-CPAP transfected on the second day for 48 h. (B) Representative co-immunoprecipitation of Myc-EGFP-CEP120 in HYLS1−/− DLD1 cells with HYLS1-mCherry (WT or D211G) add-back. Cells were induced with doxycycline for 4 days and Myc-EGFP-CEP120 transfected on the second day for 48 h. Source data are available for this figure: SourceData FS9. Refer to the image caption for details.

Co-immunoprecipitation analysis of HYLS1 with CPAP and CEP120. (A) Representative co-immunoprecipitation of Myc-EGFP-CPAP in HYLS1−/− DLD1 cells with HYLS1-mCherry (WT or D211G) add-back. Cells were induced with doxycycline for 4 days and Myc-EGFP-CPAP transfected on the second day for 48 h. (B) Representative co-immunoprecipitation of Myc-EGFP-CEP120 in HYLS1−/− DLD1 cells with HYLS1-mCherry (WT or D211G) add-back. Cells were induced with doxycycline for 4 days and Myc-EGFP-CEP120 transfected on the second day for 48 h. Source data are available for this figure: SourceData FS9.

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