Effect of AS isoform KD on isoform and total mRNA abundance in KTC1 cells and RBM10 loss-induced deregulation of cell death and cell cycle pathways by RNAseq. (A and B) qRT-PCR for VCL, CD44, and TNC using primers bracketing the indicated targeted exon inclusion isoform (A) or constitutive exons (B) in KTC1 cells transduced with sh.Renilla or isoform-specific shRNAs. Data represent mean with SD of four replicates; statistical difference by unpaired t test (***P < 0.0001). (C and D) IPA of RNAseq of PE121410 versus PE121410-RBM10 cells. (C) Inhibition of cellular functions associated with apoptotic and autophagic cancer cell death (red). (D) Enriched canonical pathways associated with inhibition of apoptosis, cell cycle checkpoints, and RhoGDI, an inhibitor of GDP dissociation in Rho family GTPases (red). IPA: Ingenuity Pathway Analysis.