Myeloid cell infiltration in SARS-CoV-2–infected mice. (a) Flow cytometry gating strategy for Fig. 1 g; Fig. 3, d–f; Fig S1, b and e; and Fig. S2, b–d. (b) Relative percentage of different cell populations in indicated knockout mice infected with SARS-CoV-2 2 DPI (WT [light gray] nontransduced, noninfected values from single experiment, n = 2; WT [dark gray] AAV-hACE2 and not infected values from two independent experiments, n = 4; WT [blue] AAV-hACE2 and infected values from two independent experiments, n = 4; IFNAR^−/− values from three independent experiments, n = 6; and IRF3/7^−/− values from two independent experiments, n = 5. (c) Representative flow cytometry plots for Fig. 3, d and e. (d) Relative percentage of myeloid cell populations in WT mice transduced with AAV-hACE2 at 2, 4, and 7 d after SARS-CoV-2 infection (WT [light gray] nontransduced, noninfected values from single experiment, n = 2; WT [dark gray] AAV-hACE2 and not infected values from two independent experiments, n = 4; WT [blue] AAV-hACE2 and infected values from two independent experiments, n = 4, at each time point. FSC-A, forward scatter area; FSC-H, forward scatter height; FSC-W, forward scatter width; SSC-A, side scatter area; SSC-H, side scatter height; SSC-W, side scatter width.