AAV-hACE2 infection makes WT mice susceptible to SARS-CoV-2. (a) Immunofluorescence staining of ACE2 in mice transduced with AAV-hACE2, 20 d after transduction. (b) Flow cytometry gating strategy for Fig. 1 g;Fig. 3, g–i; Fig S1 c; and Fig S3, a–c. (c) Representative flow cytometry plots for Fig. 1 g T cells and NK cells. (d) Representative flow cytometry plots for Fig. 1 g myeloid cells. (e) Different macrophage populations in the lungs of SARS-CoV-2–infected control (AAV-GFP or Mock) or AAV-hACE2 mice 2 DPI by flow cytometry. Data are pooled from two independent experiments, n = 4 mice per group, and are represented as mean ± SEM. P values were calculated by two-tailed unpaired Student’st test. **, P < 0.01.(f) Sera of mice were collected 7 DPI with SARS-CoV-2, and limiting dilutions were made to measure reactivity against S1 protein of SARS-CoV-2 using ELISA. (g) Sera of mice were collected 14 DPI with SARS-CoV-2, and limiting dilutions were made to measure reactivity against S1 protein of SARS-CoV-2 using ELISA. Scale bar, 100 µm. FSC-A, forward scatter area; FSC-H, forward scatter height; FSC-W, forward scatter width; SSC-A, side scatter area; SSC-H, side scatter height; SSC-W, side scatter width.
Figure S1.

AAV-hACE2 infection makes WT mice susceptible to SARS-CoV-2. (a) Immunofluorescence staining of ACE2 in mice transduced with AAV-hACE2, 20 d after transduction. (b) Flow cytometry gating strategy for Fig. 1 g;Fig. 3, g–i; Fig S1 c; and Fig S3, a–c. (c) Representative flow cytometry plots for Fig. 1 g T cells and NK cells. (d) Representative flow cytometry plots for Fig. 1 g myeloid cells. (e) Different macrophage populations in the lungs of SARS-CoV-2–infected control (AAV-GFP or Mock) or AAV-hACE2 mice 2 DPI by flow cytometry. Data are pooled from two independent experiments, n = 4 mice per group, and are represented as mean ± SEM. P values were calculated by two-tailed unpaired Student’st test. **, P < 0.01.(f) Sera of mice were collected 7 DPI with SARS-CoV-2, and limiting dilutions were made to measure reactivity against S1 protein of SARS-CoV-2 using ELISA. (g) Sera of mice were collected 14 DPI with SARS-CoV-2, and limiting dilutions were made to measure reactivity against S1 protein of SARS-CoV-2 using ELISA. Scale bar, 100 µm. FSC-A, forward scatter area; FSC-H, forward scatter height; FSC-W, forward scatter width; SSC-A, side scatter area; SSC-H, side scatter height; SSC-W, side scatter width.

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