KLF2 is dispensable for PC entry into the BM . (A) Schematic illustration of adoptive transfer of PCs into naïve mice. SPL PCs derived from tamoxifen-treated donor B1–8^hi R26-creERT2 Klf2^+/+ or KLf2^fl/fl B cells were purified and transferred. (B) Representative FCM plots of transferred CD45.1⁺ R26-creERT2 Klf2^+/+ or Klf2^fl/fl PCs detected in BM parenchyma (CD45 i.v. negative) (left), or relative number of Klf2^+/+ or Klf2^fl/fl PCs in BM parenchyma compared with the number of transferred PCs (n = 3 or 4) at 24 h or 30 days after transfer. (C) Representative FCM histograms showing surface S1pr1 expression (left) or its gMFI in SPL, blood, or BM PCs (n = 4). Data in B and C are representative of two independent experiments (n = 3 for B and n = 4 for C, in the second experiments). Data were analyzed by two-tailed unpaired Student’s t test (C). ns, not significant. PCs, plasma cells; SPL, spleen; BM, bone marrow; gMFI, geometric mean fluorescence intensity.