Survival, repertoire, and localization of antigen-specific PCs . (A) Schematic of the experimental procedure for fate mapping of PCs. (B) The number of donor-derived tdTomato⁺ PCs in SPL (left) or BM (right) at the indicated time points after immunization. Data are representative of two independent experiments. (C) Representative FCM plots of adoptively transferred integrin β7^hi or β7^lo PCs. Upper plots (“Before transfer”): Purity of donor β7^hi or β7^lo splenic PCs. Lower plots (“24 h after transfer”): Donor PCs detected in the BM 24 h after the transfer of β7^hi or β7^lo PCs. (D) Frequency of integrin β7^hi donor PCs detected in the BM (left) and the relative number of donor PCs in the BM compared with the input number (n = 4 for each donor) (right). (E) Affinity of HA-specific antibodies that were expressed in integrin β7^hi or β7^lo SPL PCs. The affinity of mAbs generated from HA-binding β7^hi or β7^lo PCs. HA-binding β7^hi or β7^lo PCs were isolated. mAbs that are derived from two clones (HV1–50 HJ4 or HV1–76 HJ1) from mouse 1 and three clones (HV1–69 HJ2, HV1–69 HJ3, or HV14-2 HJ1) from mouse 2 were generated, and their affinity against HA was measured. (F) Immunofluorescence analysis of Klf2-sufficient (+/+) or Klf2-deficient (fl/fl) PCs (CD45.1⁺CD138⁺ cells) in SPL. Experimental design is shown in Fig. 2 A. (G) gMFI of CXCR4 or CXCR5 on Klf2-sufficient (n = 6) or Klf2-deficient PCs (n = 6) in spleen. Data were analyzed by two-tailed unpaired Student’s t test. ns., not significant. Data are representative of two independent experiments. PCs, plasma cells; SPL, spleen; BM, bone marrow; gMFI, geometric mean fluorescence intensity.