The ZMIZ1-MYB network of BMP-like enhancers is essential for population growth. (A–C) qRT-PCR analysis for MYB (A), BCL2 (B), and MEF2C (C) of LOUCY, CUTLL3, and THP-6 cells that were transduced with DOX-inducible dCas9-KRAB, Tet3G, and indicated sgRNA; and treated with DOX for 3 days; n = 3/group. (D–L) Growth assay of LOUCY cells (D, G, and J), CUTLL3 cells (E, H, and K), and THP-6 cells (F, I, and L) that were transduced with DOX-inducible dCas9-KRAB, Tet3G, and sgRNA directed against the MYB enhancer (D–F), BCL2 enhancer (G–I), and MEF2C enhancer (J–L); and treated with DOX. Fold expansion = trypan blue-negative cell counts normalized to day 0 trypan blue-negative cell counts (starting on day 3 after DOX addition). Negative sgRNA control (−) = non-targeting. Positive essential sgRNA control (+) = RPL34; n = 3/group. P values were based on one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.