Figure 8.
ZMIZ1 induces MYB to directly and convergently activate BMP-like oncogenes through native stem cell enhancers. (A–C) ChIP-seq, HiChIP, and ATAC-seq tracks in LOUCY (BMP-like model), THP-6 (near ETP-ALL model), and primary ETP-ALL cells within the TADs that contain MYB (A), BCL2 (B), and MEF2C (C). Red boxes highlight putative enhancers at +140 kb of MYB (A), +220 kb of BCL2 (B), and −1 kb of MEF2C (C). GSE134761; GSE165209; GSE94000; GSE138660; GSE76783. (D) Zoomed-in ChIP-seq and ATAC-seq tracks showing co-occupancy of ZMIZ1, MYB, and MEF2C at the +140 kb MYB, +540 kb MYCN, +220 kb BCL2, and −1 kb MEF2C enhancers in LOUCY cells. (E–G) ATAC-seq profiles of the +140 kb MYB (E), +220 kb BCL2 (F), and −1 kb MEF2C (G) stem cell enhancers in sorted human CD34+Lin− cord blood cells (HSPC) and thymocytes from two donors (D1 and D2) as in Fig. 5 G. Refer to the image caption for details.

ZMIZ1 induces MYB to directly and convergently activate BMP-like oncogenes through native stem cell enhancers. (A–C) ChIP-seq, HiChIP, and ATAC-seq tracks in LOUCY (BMP-like model), THP-6 (near ETP-ALL model), and primary ETP-ALL cells within the TADs that contain MYB (A), BCL2 (B), and MEF2C (C). Red boxes highlight putative enhancers at +140 kb of MYB (A), +220 kb of BCL2 (B), and −1 kb of MEF2C (C). GSE134761; GSE165209; GSE94000; GSE138660; GSE76783. (D) Zoomed-in ChIP-seq and ATAC-seq tracks showing co-occupancy of ZMIZ1, MYB, and MEF2C at the +140 kb MYB, +540 kb MYCN, +220 kb BCL2, and −1 kb MEF2C enhancers in LOUCY cells. (E–G) ATAC-seq profiles of the +140 kb MYB (E), +220 kb BCL2 (F), and −1 kb MEF2C (G) stem cell enhancers in sorted human CD34+Lin− cord blood cells (HSPC) and thymocytes from two donors (D1 and D2) as in Fig. 5 G.

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