Figure 6.
The NMRE is not essential for the development of normal ETPs or other hematopoietic progenitors. (A) ChIP-seq profiles of leukemia-associated transcription factors at the NMRE locus in transformed murine cell lines: GSM552233 (Fli1), GSM552234 (Gata2), GSM552237 (Lmo2), GSM552238 (Lyl1), GSM552239 (Meis1), GSM552241 (Runx1), and ENCSR000ESE (Zmiz1). (B) DNA electrophoresis showing homozygous (Δ/Δ) or heterozygous (Δ/+) deletion of an 865bp NMRE fragment obtained by crossing Vav1-Cre mice with conditional NMRE-deficient mice. (C) Virtual 4-C tracks at the Mycn locus representing the loops identified by Hi-C in sorted LSKs from NMREΔ/Δ (n = 3) and NMRE+/+ (n = 3) mice. The red vertical line indicates the location of the NMRE. (D) qRT-PCR for Mycn expression in LT-HSC (Lin−Sca-1+Kit+CD150+CD48−) sorted from Mycn-deficient mice described in B. (E–H) Recipient mice received NMRE WT (n = 4) and KO (n = 5) CD45.2+ BM, competing with WT CD45.1+ cells and analyzed at 8 mo from transplantation; n = 4 and 5/group. (E) Peripheral blood chimerism of all blood cells over time. (F) Peripheral blood chimerism of T-cell, myeloid, and B-cell compartments at 5 mo from transplantation. (G) Chimerism of BMP and ETP compartments at sacrifice, 8 mo from transplantation. (H) Representative flow cytometry plots to detect chimerism of ETP compartment. Source data are available for this figure: SourceData F6. Refer to the image caption for details.

The NMRE is not essential for the development of normal ETPs or other hematopoietic progenitors. (A) ChIP-seq profiles of leukemia-associated transcription factors at the NMRE locus in transformed murine cell lines: GSM552233 (Fli1), GSM552234 (Gata2), GSM552237 (Lmo2), GSM552238 (Lyl1), GSM552239 (Meis1), GSM552241 (Runx1), and ENCSR000ESE (Zmiz1). (B) DNA electrophoresis showing homozygous (Δ/Δ) or heterozygous (Δ/+) deletion of an 865bp NMRE fragment obtained by crossing Vav1-Cre mice with conditional NMRE-deficient mice. (C) Virtual 4-C tracks at the Mycn locus representing the loops identified by Hi-C in sorted LSKs from NMREΔ/Δ (n = 3) and NMRE+/+ (n = 3) mice. The red vertical line indicates the location of the NMRE. (D) qRT-PCR for Mycn expression in LT-HSC (Lin−Sca-1+Kit+CD150+CD48−) sorted from Mycn-deficient mice described in B. (E–H) Recipient mice received NMRE WT (n = 4) and KO (n = 5) CD45.2+ BM, competing with WT CD45.1+ cells and analyzed at 8 mo from transplantation; n = 4 and 5/group. (E) Peripheral blood chimerism of all blood cells over time. (F) Peripheral blood chimerism of T-cell, myeloid, and B-cell compartments at 5 mo from transplantation. (G) Chimerism of BMP and ETP compartments at sacrifice, 8 mo from transplantation. (H) Representative flow cytometry plots to detect chimerism of ETP compartment. Source data are available for this figure: SourceData F6.

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