The Zmiz1 target gene Mycn is important for normal ETP generation. (A) RNA-seq expression of Mycn (red) and other ETP-TF5 genes in sorted murine thymocytes (Immgen). (B–K) For the experiment described in Fig. 4 G, %CD45.2⁺ chimerism was measured of peripheral blood CD4 (B) and CD8 (C) compartments over time as mean ± SEM; HSC (D, Lin⁻ Sca-1⁺Kit⁺Flt3⁻), LMPP (E, Lin⁻Sca-1⁺Kit⁺Flt3^hi), CLP-T (F, Lin⁻ Sca-1^loKit^loIl7r⁺Flt3⁺Ly6d⁻), CLP (G, Lin⁻Sca1^loKit^loIl7r⁺Flt3⁺Ly6d⁺), immature single positive (ISP) (H, CD8⁺TCRβ⁻), DP (I, CD4⁺CD8⁺), CD4 SP (J, CD4⁺CD8⁻TCRβ⁺), and CD8 SP (K, CD4⁻CD8⁺TCRβ⁺) compartments at 16 wk after transplantation; n = 10 (control) and 7 (knockout)/group. (L)Mycn qRT-PCR of sorted ETP and DN2 cells from Il7rCre control and Il7Cre Mycn^f/f mice; n = 3/group. P values were based on two-sided t test. *P < 0.05; **P < 0.01; ***P < 0.001.