ZMIZ1 and MYB promote the expression of BMP-like oncogenes in normal and malignant ETPs. (A) Western blot of NOTCH1-derived polypeptides in LOUCY cells shows that the NOTCH1 receptor is inactive but poised to activate when engaged with ligand DLL4. (B) Volcano plot of significance versus log₂FC of RNA-seq data between shControl and shZMIZ1-13 transduced LOUCY cells; n = 4/group. (C–F) qRT-PCR validation of the ETP-ALL oncogenes highlighted in Fig. 3 A; n = 4/group. (G) HOMER de novo motif analyses of ZMIZ1, MYB, and MEF2C ChIP-seq peaks showing the top seven motifs ranked by P value. (H–J) Motif analyses of MYB and ZMIZ1 peaks grouped as MYB alone (H), ZMIZ1 alone (I), and overlapping peaks (J). (K–M) Motif analyses of MEF2C and ZMIZ1 peaks grouped as MEF2C alone (K), ZMIZ1 alone (L), and overlapping peaks (M). (N–Q) qRT-PCR validation of the BMP-like oncogenes highlighted in Fig. 3 L; n = 4/group. (R) Venn diagram showing overlap of ZMIZ1- and MYB-repressed genes. P values are for both Fisher exact test and Chi-square. Unless noted otherwise, P values were based on one-way ANOVA. **P < 0.01; ***P < 0.001. Source data are available for this figure: SourceData FS3.