Figure 7.
The MLL3/GRHL2-IRF1 axis regulates the expression of chemokines CXCL9 and CXCL10. (A–C) Relative mRNA expression levels of CXCL9/10 in indicated cell line and organoid samples upon silencing of either MLL3 or GRHL2 in the presence of IFNγ (10 ng/ml). (D) Protein levels of IRF1 after overexpression of IRF1. (E) mRNA expression levels of CXCL9 and CXCL10 upon overexpression of IRF1. (F) ELISA assay of CXCL9/10 upon knockdown of either MLL3 or GRHL2 in the absence or presence of IFNγ (10 ng/ml). (G) IRF1 ChIP-qPCR in the absence or presence of IFNγ (10 ng/ml). n = 3 biological replicates for A–C and E–G. *P < 0.05; **P < 0.01; and ***P < 0.001. Source data are available for this figure: SourceData F7. Refer to the image caption for details.

The MLL3/GRHL2-IRF1 axis regulates the expression of chemokines CXCL9 and CXCL10. (A–C) Relative mRNA expression levels of CXCL9/10 in indicated cell line and organoid samples upon silencing of either MLL3 or GRHL2 in the presence of IFNγ (10 ng/ml). (D) Protein levels of IRF1 after overexpression of IRF1. (E) mRNA expression levels of CXCL9 and CXCL10 upon overexpression of IRF1. (F) ELISA assay of CXCL9/10 upon knockdown of either MLL3 or GRHL2 in the absence or presence of IFNγ (10 ng/ml). (G) IRF1 ChIP-qPCR in the absence or presence of IFNγ (10 ng/ml). n = 3 biological replicates for A–C and E–G. *P < 0.05; **P < 0.01; and ***P < 0.001. Source data are available for this figure: SourceData F7.

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