MLL3/GRHL2 knockdown accelerates tumor growth and reduces T cell infiltration. (A) Kaplan–Meier survival plots of control and MLL3-knockdown groups in MOC22 xenografts models (n = 3 biological replicates). Mice were sacrificed when tumor reached 1.5 cm. (B) Gating strategy for flow cytometry of Fig. 5, B and D. The cell population was first gated on SSC-A/FSC-A and FSC-H/FSC-A parameters, and then Zombie Violet dye was used to exclude dead cells. Next, CD45⁺CD3⁺-positive T cells were gated to isolate the T cell population. Finally, we measured the proportions of CD8⁺ and CD4⁺ T cells using the following equation: $CD8+T cell(%)=Number of CD8+CD4− T cellsNumber of live cells×100$ and $CD4+ T cell (%)=Number of CD8−CD4+T cellsNumber of live cells×100$⁠.