MLL3 and GRHL2 co-bind large numbers of genomic loci and regulate immune response pathways. (A) Quantification of colonies after knockdown of MLL3 (n = 5 biological replicates) in A253 and FADU cell lines. (B) Pie charts showing genomic distribution of MLL3- and GRHL2-binding region in KYSE510 cell line. (C) Heatmap of ChIP-seq peaks of MLL3, GRHL2, H3K27ac, and H3K4me1. (D and E) Gene set enrichment analysis (GSEA) of either MLL3 KD or GRHL2 KD RNA-seq. *P < 0.05; **P < 0.01; and ***P < 0.001.