Figure 3.
MLL3 inhibits cancer cell proliferation and interacts with GRHL2. (A) Protein levels of MLL3 following siRNA transfection for 48 h in KYSE510 and KYSE180 cell lines. (B) Quantification of colonies measured by absorbance rate of 480 nm (n = 5 biological replicates). (C) Protein levels of MLL3 after viral infection of shRNA into KYSE510 and KYSE180 cell lines. (D) Quantification of colonies in MLL3-silenced versus control KYSE510 and KYSE180 cell lines (n = 5 biological replicates). (E and F) Relative mRNA and protein levels of MLL3 following its knockdown in MOC1 and MOC22 cell lines, measured by qRT-PCR and western blot, respectively. (G) Quantification of colonies in MLL3-silenced MOC1 and MOC22 cell lines (n = 5 biological replicates). (H) Table of top enriched transcription factor–binding motifs in MLL3 ChIP-seq peak regions. (I) Venn diagram displaying the number of overlapped peaks between GRHL2 and MLL3 ChIP-seq in KYSE510 cell line. (J) Co-IP assay by pulling down using the anti-MLL3 antibody followed by detection of GRHL2 protein by western blot. *P < 0.05; **P < 0.01; ***P < 0.001. Co-IP: co-immunoprecipitation. Source data are available for this figure: SourceData F3. Refer to the image caption for details.

MLL3 inhibits cancer cell proliferation and interacts with GRHL2. (A) Protein levels of MLL3 following siRNA transfection for 48 h in KYSE510 and KYSE180 cell lines. (B) Quantification of colonies measured by absorbance rate of 480 nm (n = 5 biological replicates). (C) Protein levels of MLL3 after viral infection of shRNA into KYSE510 and KYSE180 cell lines. (D) Quantification of colonies in MLL3-silenced versus control KYSE510 and KYSE180 cell lines (n = 5 biological replicates). (E and F) Relative mRNA and protein levels of MLL3 following its knockdown in MOC1 and MOC22 cell lines, measured by qRT-PCR and western blot, respectively. (G) Quantification of colonies in MLL3-silenced MOC1 and MOC22 cell lines (n = 5 biological replicates). (H) Table of top enriched transcription factor–binding motifs in MLL3 ChIP-seq peak regions. (I) Venn diagram displaying the number of overlapped peaks between GRHL2 and MLL3 ChIP-seq in KYSE510 cell line. (J) Co-IP assay by pulling down using the anti-MLL3 antibody followed by detection of GRHL2 protein by western blot. *P < 0.05; **P < 0.01; ***P < 0.001. Co-IP: co-immunoprecipitation. Source data are available for this figure: SourceData F3.

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