Effect of palmitoylation on membrane targeting by ABHD17A in CG simulations. (A) Mapping of palmitoylated AA ABHD17A full length to CG MARTINI3.0 model, with four palmitoyl chains at its N-terminus. (B) Minimum distance analysis of CG-MD simulations of palmitoylated ABHD17A. In all four independent replicas, the protein shows association with the nearby membrane, on a μs timescale. (C and D) Membrane interaction of the palmitoylated N-terminal helix and conserved loop shown in the contacts analysis (C) and the occupancy maps (D).