Aβ plaque burden in Atg7 ^fl/fl -5xFAD and Atg7 ^ΔMG -5xFAD mice. (A) Representative images showing Methoxy-X04 staining in the cortex and hippocampus of 5-mo-old Atg7^fl/fl-5xFAD and Atg7^ΔMG-5xFAD mice. Scale bar = 500 μm. (B) Quantification of the percentage of Methoxy-X04⁺ area within the cortex of Atg7^fl/fl-5xFAD and Atg7^ΔMG-5xFAD mice. Each data point represents data from one mouse with two technical repeats. ns = not significant by unpaired t test. Data are presented as mean ± SEM. (C) Quantification of insoluble Aβ_1–42 in the cortex lysates from 5- and 12-mo-old Atg7^fl/fl-5xFAD and Atg7^ΔMG-5xFAD mice. Each data point represents one mouse with three technical repeats. ns = not significant by unpaired t test. Data are presented as mean ± SEM. (D) Representative zoomed in confocal images showing the co-staining of IBA1 and LAMP1. Left: White arrow show LAMP1⁺ lysosomal vesicles within microglial cytosol. Right: White arrow show microglia staining that is not overlapped with LAMP1⁺ dystrophic neurites. Scale bar = 30 μm.